Description: Rabbit polyclonal antibody to MARS2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human MARS2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MARS2 Alternative Names: Methionine--tRNA ligase, mitochondrial; Methionyl-tRNA synthetase 2; Mitochondrial methionyl-tRNA synthetase; MtMetRSEntrez Gene (Human): 92935Entrez Gene (Mouse) : 212679SwissProt (Human): Q96GW9SwissProt (Mouse) : Q499X9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MARS2 expression in HL60 (A) whole cell lysates.
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  Immunohistochemical analysis of MARS2 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.