Description: Rabbit polyclonal antibody to Semaphorin 3A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human Semaphorin 3A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SEMA3A Alternative Names: SEMAD; Semaphorin-3A; Semaphorin III; Sema IIIEntrez Gene (Human): 10371SwissProt (Human): Q14563Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Semaphorin 3A expression in CEM (A) whole cell lysates.
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Immunohistochemical analysis of Semaphorin 3A staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.