Description: Rabbit polyclonal antibody to CASC3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human CASC3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CASC3 Alternative Names: MLN51; Protein CASC3; Cancer susceptibility candidate gene 3 protein; Metastatic lymph node gene 51 protein; MLN 51; Protein barentsz; BtzEntrez Gene (Human): 22794SwissProt (Human): O15234Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of CASC3 expression in NCIH460 (A) whole cell lysates.
-
Immunohistochemical analysis of CASC3 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.