Description: Rabbit polyclonal antibody to CaMK1 alpha Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CaMK1 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CAMK1 Alternative Names: Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alphaEntrez Gene (Human): 8536Entrez Gene (Mouse) : 52163Entrez Gene (Rat) : 171503SwissProt (Human): Q14012SwissProt (Mouse) : Q91YS8SwissProt (Rat) : Q63450Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CaMK1 alpha expression in HeLa (A), human primate brain (B) whole cell lysates.
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Immunohistochemical analysis of CaMK1 alpha staining in human hepatocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.