Description: Rabbit polyclonal antibody to PKC eta Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human PKC eta. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PRKCH Alternative Names: PKCL; PRKCL; Protein kinase C eta type; PKC-L; nPKC-etaEntrez Gene (Human): 5583SwissProt (Human): P24723Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PKC eta expression in A431 (A), HL60 (B), NCIH460 (C) whole cell lysates.
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  Immunohistochemical analysis of PKC eta staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.