Description: Rabbit polyclonal antibody to RCN3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human RCN3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50) Gene Symbol: RCN3 Alternative Names: Reticulocalbin-3; EF-hand calcium-binding protein RLP49Entrez Gene (Human): 57333Entrez Gene (Mouse) : 52377SwissProt (Human): Q96D15SwissProt (Mouse) : Q8BH97Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RCN3 expression in U251MG (A), U2OS (B), A549 (C), HepG2 (D), mouse lung (E) whole cell lysates.
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Flow cytometric analysis of CEM cells using Anti-RCN3 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.