Description: Rabbit polyclonal antibody to N4BP3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human N4BP3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: N4BP3 Alternative Names: KIAA0341; NEDD4-binding protein 3; N4BP3Entrez Gene (Human): 23138SwissProt (Human): O15049Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of N4BP3 expression in ZR751 (A) whole cell lysates.
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Immunohistochemical analysis of N4BP3 staining in human cervix carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.