Description: Rabbit polyclonal antibody to RBCK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human RBCK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RBCK1 Alternative Names: C20orf18; RNF54; UBCE7IP3; XAP3; XAP4; RanBP-type and C3HC4-type zinc finger-containing protein 1; HBV-associated factor 4; Heme-oxidized IRP2 ubiquitin ligase 1; HOIL-1; Hepatitis B virus X-associated protein 4; RING finger protein 54; Ubiquitin-conjugating enzyme 7-interacting protein 3Entrez Gene (Human): 10616Entrez Gene (Mouse) : 24105SwissProt (Human): Q9BYM8SwissProt (Mouse) : Q9WUB0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RBCK1 expression in mouse kidney (A) whole cell lysates.
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  Immunohistochemical analysis of RBCK1 staining in human bladder carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.