Description: Rabbit polyclonal antibody to CHST12 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human CHST12. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CHST12 Alternative Names: Carbohydrate sulfotransferase 12; Chondroitin 4-O-sulfotransferase 2; Chondroitin 4-sulfotransferase 2; C4ST-2; C4ST2; Sulfotransferase HloEntrez Gene (Human): 55501SwissProt (Human): Q9NRB3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CHST12 expression in HL60 (A) whole cell lysates.
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Immunohistochemical analysis of CHST12 staining in human small intestine formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.