Description: Rabbit polyclonal antibody to SKOR2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human SKOR2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: SKOR2 Alternative Names: CORL2; FUSSEL18; SKI family transcriptional corepressor 2; Functional Smad-suppressing element on chromosome 18; Fussel-18; LBX1 corepressor 1-like protein; Ladybird homeobox corepressor 1-like proteinEntrez Gene (Human): 652991Entrez Gene (Mouse) : 664805SwissProt (Human): Q2VWA4SwissProt (Mouse) : A7M7C7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SKOR2 expression in human cerebellum (A), human testis (B), mouse cerebellum (C) whole cell lysates.
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Immunofluorescent analysis of Anti-SKOR2 staining in mouse cerebellum cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).