Description: Rabbit polyclonal antibody to GDF11 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human GDF11. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GDF11 Alternative Names: BMP11; Growth/differentiation factor 11; GDF-11; Bone morphogenetic protein 11; BMP-11Entrez Gene (Human): 10220Entrez Gene (Mouse) : 14561SwissProt (Human): O95390SwissProt (Mouse) : Q9Z1W4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GDF11 expression in 293 (A), human heart (B), Hela (C), K562 (D), mouse brain (E), mouse kidney (F) whole cell lysates.
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Immunohistochemical analysis of GDF11 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.