Description: Rabbit polyclonal antibody to Siglec 11 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human Siglec 11. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SIGLEC11 Alternative Names: Sialic acid-binding Ig-like lectin 11; Sialic acid-binding lectin 11; Siglec-11Entrez Gene (Human): 114132SwissProt (Human): Q96RL6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Siglec 11 expression in HepG2 (A) whole cell lysates.
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Immunohistochemical analysis of Siglec 11 staining in human lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.