Description: Rabbit polyclonal antibody to CD327 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CD327. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SIGLEC6 Alternative Names: CD33L; CD33L1; OBBP1; Sialic acid-binding Ig-like lectin 6; Siglec-6; CD33 antigen-like 1; CDw327; Obesity-binding protein 1; OB-BP1; CD327Entrez Gene (Human): 946SwissProt (Human): O43699Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD327 expression in mouse spleen (A) whole cell lysates.
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Immunohistochemical analysis of CD327 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.