Description: Rabbit polyclonal antibody to NR1D2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human NR1D2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: NR1D2 Alternative Names: Nuclear receptor subfamily 1 group D member 2; Orphan nuclear hormone receptor BD73; Rev-erb alpha-related receptor; RVR; Rev-erb-beta; V-erbA-related protein 1-related; EAR-1REntrez Gene (Human): 9975SwissProt (Human): Q14995Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NR1D2 expression in Hela (A) whole cell lysates.
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Immunohistochemical analysis of NR1D2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.