Description: Rabbit polyclonal antibody to POTED Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human POTED. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: POTED Alternative Names: A26B3; ANKRD21; POTE; POTE ankyrin domain family member D; ANKRD26-like family B member 3; Ankyrin repeat domain-containing protein 21; Prostate, ovary, testis-expressed protein; Protein POTEEntrez Gene (Human): 100288966;317754SwissProt (Human): Q86YR6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of POTED expression in human ovary (A), human testis (B) whole cell lysates.
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Immunohistochemical analysis of POTED staining in human prostata carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.