Description: Rabbit polyclonal antibody to CD163b Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CD163b. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CD163L1 Alternative Names: CD163B; M160; Scavenger receptor cysteine-rich type 1 protein M160; CD163 antigen-like 1; CD163bEntrez Gene (Human): 283316SwissProt (Human): Q9NR16Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD163b expression in U2OS (A) whole cell lysates.
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Immunohistochemical analysis of CD163b staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.