Description: Rabbit polyclonal antibody to SPATA18 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human SPATA18. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SPATA18 Alternative Names: MIEAP; Mitochondria-eating protein; Spermatogenesis-associated protein 18Entrez Gene (Human): 132671Entrez Gene (Mouse) : 73472SwissProt (Human): Q8TC71SwissProt (Mouse) : Q0P557Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SPATA18 expression in NCIH292 (A) whole cell lysates.
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Immunohistochemical analysis of SPATA18 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.