Description: Rabbit polyclonal antibody to NSMAF Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human NSMAF. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: NSMAF Alternative Names: FAN; Protein FAN; Factor associated with neutral sphingomyelinase activation; Factor associated with N-SMase activationEntrez Gene (Mouse) : 18201SwissProt (Mouse) : O35242Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NSMAF expression in mouse cerebellum (A) whole cell lysates.
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  Immunohistochemical analysis of NSMAF staining in human thyroid formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.