Description: Rabbit polyclonal antibody to PTPLA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human PTPLA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PTPLA Alternative Names: HACD1; Very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 1; 3-hydroxyacyl-CoA dehydratase 1; HACD1; Cementum attachment protein; Protein-tyrosine phosphatase-like member AEntrez Gene (Human): 9200SwissProt (Human): B0YJ81Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PTPLA expression in MDAMB231 (A) whole cell lysates.
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Immunohistochemical analysis of PTPLA staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.