Description: Rabbit polyclonal antibody to USP45 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human USP45. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: USP45 Alternative Names: Ubiquitin carboxyl-terminal hydrolase 45; Deubiquitinating enzyme 45; Ubiquitin thioesterase 45; Ubiquitin-specific-processing protease 45Entrez Gene (Human): 85015Entrez Gene (Mouse) : 77593SwissProt (Human): Q70EL2SwissProt (Mouse) : Q8K387Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of USP45 expression in A549 (A) whole cell lysates.
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  Immunohistochemical analysis of USP45 staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.