Description: Rabbit polyclonal antibody to B3GNT1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human B3GNT1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: B3GNT1 Alternative Names: B3GNT6; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; I-beta-1,3-N-acetylglucosaminyltransferase; iGnT; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1Entrez Gene (Human): 11041SwissProt (Human): O43505Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of B3GNT1 expression in MDAMB435 (A) whole cell lysates.
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Immunohistochemical analysis of B3GNT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.