Description: Rabbit polyclonal antibody to SmgGDS Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human SmgGDS. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RAP1GDS1 Alternative Names: Rap1 GTPase-GDP dissociation stimulator 1; Exchange factor smgGDS; SMG GDS protein; SMG P21 stimulatory GDP/GTP exchange proteinEntrez Gene (Human): 5910SwissProt (Human): P52306Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SmgGDS expression in HepG2 (A), MDAMB231 (B) whole cell lysates.
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Immunohistochemical analysis of SmgGDS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.