Description: Rabbit polyclonal antibody to TUBB2B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human TUBB2B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: TUBB2B Alternative Names: Tubulin beta-2B chainEntrez Gene (Human): 347733Entrez Gene (Mouse) : 73710Entrez Gene (Rat) : 291081SwissProt (Human): Q9BVA1SwissProt (Mouse) : Q9CWF2SwissProt (Rat) : Q3KRE8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TUBB2B expression in Hela (A), HepG2 (B), MDAMB231 (C), K562 (D), mouse brain (E), mouse cerebellum (F), PC12 (G) whole cell lysates.
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  Immunohistochemical analysis of TUBB2B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.