Description: Rabbit polyclonal antibody to CCDC36 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CCDC36. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50) Gene Symbol: CCDC36 Alternative Names: Coiled-coil domain-containing protein 36; Cancer/testis antigen 74; CT74Entrez Gene (Human): 339834SwissProt (Human): Q8IYA8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CCDC36 expression in 293 (A), Neuro2a (B), mouse liver (C) whole cell lysates.
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  Immunohistochemical analysis of CCDC36 staining in human esophageal carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Anti-CCDC36 staining in 293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).