Description: Rabbit polyclonal antibody to Siglec 15 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Siglec 15. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SIGLEC15 Alternative Names: CD33L3; Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3Entrez Gene (Human): 284266SwissProt (Human): Q6ZMC9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Siglec 15 expression in K562 (A) whole cell lysates.
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Immunohistochemical analysis of Siglec 15 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.