Description: Rabbit polyclonal antibody to GAGE12B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human GAGE12B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GAGE12B Alternative Names: G antigen 12B; GAGE-12BEntrez Gene (Human): 100132399;729422;729428;729431SwissProt (Human): A1L429Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GAGE12B expression in T47D (A) whole cell lysates.
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Immunohistochemical analysis of GAGE12B staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.