Description: Rabbit polyclonal antibody to ACAP1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human ACAP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ACAP1 Alternative Names: CENTB1; KIAA0050; Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; Centaurin-beta-1; Cnt-b1Entrez Gene (Human): 9744Entrez Gene (Mouse) : 216859SwissProt (Human): Q15027SwissProt (Mouse) : Q8K2H4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ACAP1 expression in ZR751 (A) whole cell lysates.
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Immunohistochemical analysis of ACAP1 staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.