Description: Rabbit polyclonal antibody to IFITM5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human IFITM5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), FC (1/10 - 1/30) Gene Symbol: IFITM5 Alternative Names: Interferon-induced transmembrane protein 5; Bone-restricted interferon-induced transmembrane protein-like protein; BRIL; Dispanin subfamily A member 1; DSPA1Entrez Gene (Human): 387733Entrez Gene (Mouse) : 73835SwissProt (Human): A6NNB3SwissProt (Mouse) : O88728Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of IFITM5 expression in T47D (A), PC3 (B), SW480 (C), HT29 (D), PANC1 (E) whole cell lysates.
-
Flow cytometric analysis of A431 cells using Anti-IFITM5 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.