Description: Rabbit polyclonal antibody to CD37 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human CD37. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50) Gene Symbol: CD37 Alternative Names: TSPAN26; Leukocyte antigen CD37; Tetraspanin-26; Tspan-26; CD37Entrez Gene (Human): 951SwissProt (Human): P11049Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of CD37 expression in Jurkat (A), HCT116 (B) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 31 kD)
-
Flow cytometric analysis of CEM cells using Anti-CD37 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.