Description: Rabbit polyclonal antibody to SPATC1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human SPATC1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SPATC1 Alternative Names: SPATA15; SPRN; Speriolin; Spermatogenesis and centriole-associated protein 1; Spermatogenesis-associated protein 15; Spermatogenic cell-specific Cdc20-binding proteinEntrez Gene (Human): 375686Entrez Gene (Mouse) : 74281SwissProt (Human): Q76KD6SwissProt (Mouse) : Q148B6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SPATC1 expression in HepG2 (A), human testis (B) whole cell lysates.
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Immunohistochemical analysis of SPATC1 staining in human testis carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.