Description: Rabbit polyclonal antibody to CRIP3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human CRIP3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CRIP3 Alternative Names: CRP3; Cysteine-rich protein 3; CRP-3; Chromosome 6 LIM domain only protein; h6LIMoEntrez Gene (Human): 401262SwissProt (Human): Q6Q6R5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CRIP3 expression in U251MG (A) whole cell lysates.
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Immunohistochemical analysis of CRIP3 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.