Description: Rabbit polyclonal antibody to AP3S1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human AP3S1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: AP3S1 Alternative Names: CLAPS3; AP-3 complex subunit sigma-1; AP-3 complex subunit sigma-3A; Adapter-related protein complex 3 subunit sigma-1; Clathrin-associated/assembly/adapter protein, small 3; Sigma-3A-adaptin; Sigma3A-adaptin; Sigma-adaptin 3aEntrez Gene (Human): 1176Entrez Gene (Mouse) : 11777SwissProt (Human): Q92572SwissProt (Mouse) : Q9DCR2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AP3S1 expression in A2058 (A) whole cell lysates.
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Immunohistochemical analysis of AP3S1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.