Description: Rabbit polyclonal antibody to ATP6V0B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human ATP6V0B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ATP6V0B Alternative Names: ATP6F; V-type proton ATPase 21 kDa proteolipid subunit; V-ATPase 21 kDa proteolipid subunit; Vacuolar proton pump 21 kDa proteolipid subunit; hATPLEntrez Gene (Human): 533SwissProt (Human): Q99437Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATP6V0B expression in U251MG (A) whole cell lysates.
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  Immunohistochemical analysis of ATP6V0B staining in human prostata carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.