Description: Rabbit polyclonal antibody to PEMT Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human PEMT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PEMT Alternative Names: PEMPT; PNMT; Phosphatidylethanolamine N-methyltransferase; PEAMT; PEMT; PEMT2Entrez Gene (Human): 10400Entrez Gene (Mouse) : 18618SwissProt (Human): Q9UBM1SwissProt (Mouse) : Q61907Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PEMT expression in CEM (A), rat L6 (B), human liver (C) whole cell lysates.
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  Immunohistochemical analysis of PEMT staining in human hepatocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.