Description: Rabbit polyclonal antibody to Hevin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human Hevin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SPARCL1 Alternative Names: SPARC-like protein 1; High endothelial venule protein; Hevin; MAST 9Entrez Gene (Human): 8404SwissProt (Human): Q14515Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Hevin expression in NCIH460 (A) whole cell lysates.
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  Immunohistochemical analysis of Hevin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.