Description: Rabbit polyclonal antibody to NIPAL4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human NIPAL4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: NIPAL4 Alternative Names: ICHN; NIPA4; Magnesium transporter NIPA4; Ichthyin; NIPA-like protein 4; Non-imprinted in Prader-Willi/Angelman syndrome region protein 4Entrez Gene (Human): 348938Entrez Gene (Mouse) : 214112SwissProt (Human): Q0D2K0SwissProt (Mouse) : Q8BZF2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NIPAL4 expression in human brain (A) whole cell lysates.
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Immunohistochemical analysis of NIPAL4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.