Description: Rabbit polyclonal antibody to CTDSPL Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human CTDSPL. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50) Gene Symbol: CTDSPL Alternative Names: C3orf8; NIF1; NIFL; SCP3; YA22; CTD small phosphatase-like protein; CTDSP-like; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3; NIF-like protein; Nuclear LIM interactor-interacting factor 1; NLI-interacting factor 1; Protein YA22; hYA22; RBSP3; Small C-terminal domain phosphatase 3; SCP3; Small CTD phosphatase 3Entrez Gene (Human): 10217SwissProt (Human): O15194Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CTDSPL expression in Hela (A), K562 (B) whole cell lysates.
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Flow cytometric analysis of Hela cells using Anti-CTDSPL Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.