Description: Rabbit polyclonal antibody to TC2N Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human TC2N. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: TC2N Alternative Names: C14orf47; MTAC2D1; Tandem C2 domains nuclear protein; Membrane targeting tandem C2 domain-containing protein 1; Tandem C2 protein in nucleus; Tac2-NEntrez Gene (Human): 123036Entrez Gene (Mouse) : 74413SwissProt (Human): Q8N9U0SwissProt (Mouse) : Q91XT6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TC2N expression in NIH3T3 (A) whole cell lysates.
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Immunohistochemical analysis of TC2N staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.