Description: Rabbit polyclonal antibody to CHAT Immunogen: Recombinant fusion protein of human CHAT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: CHAT Alternative Names: Choline O-acetyltransferase; CHOACTase; ChAT; Choline acetylaseEntrez Gene (Human): 1103SwissProt (Human): P28329SwissProt (Mouse) : Q03059SwissProt (Rat) : P32738Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CHAT expression in HepG2 (A), HeLa (B), C6 (C) whole cell lysates.
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Immunofluorescent analysis of Integrin alpha 7 staining in rat brain. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).