Description: Mouse monoclonal antibody to Histone H2A.X (Phospho-S139) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S139 of human Histone H2A.X protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: H2AX Alternative Names: H2AX; Histone H2AX; H2a/x; Histone H2A.XEntrez Gene (Human): 3014Entrez Gene (Mouse) : 15270SwissProt (Human): P16104SwissProt (Mouse) : P27661Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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WesteWestern blot analysis of Histone H2A.X (Phospho-S139) expression in NIH3T3 treated with Hydroxyurea (A) whole cell lysates.rn blot analysis of Histone H2A.X (pS139) expression in NIH3T3 treated with Hydroxyurea (A) whole cell lysates.
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Immunofluorescent analysis of Histone H2A.X (Phospho-S139) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.