Description: Mouse monoclonal antibody to PCNA Immunogen: Purified recombinant human PCNA protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: PCNA Alternative Names: Proliferating cell nuclear antigen; PCNA; CyclinEntrez Gene (Human): 5111Entrez Gene (Mouse) : 18538Entrez Gene (Rat) : 25737SwissProt (Human): P12004SwissProt (Mouse) : P17918SwissProt (Rat) : P04961Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PCNA expression in Hela (A), NIH3T3 (B), COS7 (C), C6 (D), CHOK1 (E) whole cell lysates.
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  Immunohistochemical analysis of PCNA staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.