Description: Mouse monoclonal antibody to SIRT6 Immunogen: Purified recombinant human SIRT6 protein expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: SIRT6 Alternative Names: SIR2L6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6Entrez Gene (Human): 51548Entrez Gene (Mouse) : 50721SwissProt (Human): Q8N6T7SwissProt (Mouse) : P59941Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SIRT6 expression in Jurkat (A), MCF7 (B), HCT116 (C), K562 (D), COS7 (E), C2C12 (F), NIH3T3 (G) whole cell lysates.
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Immunofluorescent analysis of SIRT6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.