Description: Mouse monoclonal antibody to GSK3 beta Immunogen: Purified recombinant GSK-3β protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100) Gene Symbol: GSK3B Alternative Names: Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3BEntrez Gene (Human): 2932Entrez Gene (Mouse) : 56637Entrez Gene (Rat) : 84027SwissProt (Human): P49841SwissProt (Mouse) : Q9WV60SwissProt (Rat) : P18266Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GSK3 beta expression in A549 (A), Hela (B), NIH3T3 (C), rat brain (D) whole cell lysates.
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Immunofluorescent analysis of GSK3 beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.