Description: Mouse monoclonal antibody to CD54 Immunogen: Purified recombinant human CD54(ICAM-1) protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: ICAM1 Alternative Names: Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54Entrez Gene (Human): 3383SwissProt (Human): P05362Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD54 expression in SW480 (A), Raji (B) whole cell lysates.
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  Immunofluorescent analysis of CD54 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.