Description: Mouse monoclonal antibody to CD54 Immunogen: Purified recombinant human CD54(ICAM-1) protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ICAM1 Alternative Names: Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54Entrez Gene (Human): 3383SwissProt (Human): P05362Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD54 expression in Raji (A), SW480 (B) whole cell lysates.
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Immunohistochemical analysis of CD54 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.