Description: Mouse monoclonal antibody to CHD3 Immunogen: Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: CHD3 Alternative Names: Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFHEntrez Gene (Human): 1107SwissProt (Human): Q12873Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of CHD3 expression in NTERA2 (A), NIH3T3 (B), C6 (C), K562 (D), Hela (E) whole cell lysates.
• 
  Immunofluorescent analysis of CHD3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.