Description: Mouse monoclonal antibody to GRP78 Immunogen: Purified recombinant human BiP/GRP78 protein expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: HSPA5 Alternative Names: GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiPEntrez Gene (Human): 3309Entrez Gene (Mouse) : 14828Entrez Gene (Rat) : 25617SwissProt (Human): P11021SwissProt (Mouse) : P20029SwissProt (Rat) : P06761Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GRP78 expression in NIH3T3 (A), A549 (B), C6 (C), Hela (D) whole cell lysates.
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  Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.