Description: Mouse monoclonal antibody to GSK3 beta Immunogen: Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/50 - 1/100) Gene Symbol: GSK3B Alternative Names: Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3BEntrez Gene (Human): 2932Entrez Gene (Mouse) : 56637Entrez Gene (Rat) : 84027SwissProt (Human): P49841SwissProt (Mouse) : Q9WV60SwissProt (Rat) : P18266Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GSK3 beta expression in Hela (A), NIH3T3 (B), C6 (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of GSK3 beta staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Flow cytometric analysis of GSK3 beta in NIH3T3 cells (green) and negative control (red).