Description: Mouse monoclonal antibody to MCM2 Immunogen: Purified recombinant fragment of human MCM2 expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100) Gene Symbol: MCM2 Alternative Names: BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28Entrez Gene (Human): 4171Entrez Gene (Mouse) : 17216SwissProt (Human): P49736SwissProt (Mouse) : P97310Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MCM2 expression in Jurkat (A), K562 (B), NIH3T3 (C), Hela (D) whole cell lysates.
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Immunohistochemical analysis of MCM2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of MCM2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.