Description: Mouse monoclonal antibody to ALPP Immunogen: Purified recombinant fragment of human ALPP expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ALPP Alternative Names: PLAP; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1Entrez Gene (Human): 250SwissProt (Human): P05187Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ALPP expression in HepG2 (A), A431 (B), MCF7 (C) whole cell lysates.
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Immunohistochemical analysis of ALPP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.